It will grow either aerobically or in the absence of oxygen, but better in its presence.ĭurham tube for fermentation activity and ONPG for beta-Galactosidase activity. coli, no green-sheen.ĭeep agar for cultivating anaerobic bacteria. Gram negative results 11-15-14 Growth but not E.Gram positive results 11-15-14 Little or non bacteria culture.Lactose nonfermenters such as Salmonella, Proteus, Pseudomonas form colorless to amber colonies.It contains the dyes eosin Y and methylene blue, which partially suppress the growth of gram-positive bacteria.coli will take up the dyes and form green to blue-black colonies with a metallic sheen Staphylococcus aureus will ferment the mannitol and form yellow zones in the reddish agar because phenol red becomes yellow in the presence of fermentation acids.7.5% sodium chloride inhibits the growth of most bacteria.Hemolytic bacteria produces clear zones around their colonies because of blood cell destruction.Gram negative results 11-15-14: Hemolytic bacteria (beta?).Gram positive results 11-15-14: No hemolytic bacteria.Streptococci and staphylococci are hemolytic.It distinguishes between hemolytic and non-hemolytic bacteria.
coli produces white colonies Blood agar is selective and differential media. subtilis produces white to cram colonies E. Gram Negative: Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella arizonae, Shigella sonnei.subtilis, Enterococcus faecalis, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus. Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction.Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination.Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Tests.Proteins, Amino Acids, and Enzymes VIII: Urease Activity.Proteins, Amino Acids, and Enzymes VII: Oxidase Test and Bioluminescence.Proteins, Amino Acids, and Enzymes VI: Oxidase Test and Bioluminescence.Proteins, Amino Acids, and Enzymes V: Coagulase and DNase Activity.Proteins, Amino Acids, and Enzymes IV: Catalase Activity.Proteins, Amino Acids, and Enzymes III: Gelatin Hydrolysis.Proteins, Amino Acids, and Enzymes II: Casein Hydrolysis.Proteins, Amino Acids, and Enzymes I: The IMViC Tests.Carbohydrates II: Triple Sugar Iron Agar Test.Carbohydrates I: Fermentation and β-Galactosidase Activity.The Steak-Plate Technique: Selective and Differential Media.Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures.Sterilization and Microbiological Culture Media Preparation.Flagella Staining: West and Difco’s SpotTest Methods.Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin).Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedure.
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